Alga-PrAS

Property Search	Keyword Search	ID Search
Sequence Search	Statistics	Resources & Links

Select Species
(*Mandatory)

Species

Sequence and
Physicochemical Properties

Length

– aa

Charged

– %

Nonpolar

– %

Acidic

– %

Basic

– %

GRAVY

–

Solubility (SOLpro)

soluble insoluble

Structural Properties

β Sheet (SSpro)

– %

Disorder (DISOPRED3)

– %

Long Disorder (DISOPRED3)

Signal (SignalP)

hit not hit

Membrane (TMHMM)

N-terminal Topology (TMHMM)

S-S Bond (DIpro)

Domain Linker (DROP)

Internal Repeat (RADAR)

PEST Region (ePESTfind)

Functional Annotation

Pfam

CDD

hit not hit

UniProtKB/Swiss-Prot

hit not hit

UniProtKB/TrEMBL (Eukaryote)

hit not hit

PDB-Identities

– %

KOG

Gene Ontology

Modification and
Subcellular Localization

STY-Phosphorylation Site (Musite)

Ubiquitination Site (UbPred)

N-Glycosylation Site (NetNGlyc)

O-Glycosylation Site (Plant O-glycosylation motif)

Subcellular Localization (WoLF PSORT)

GPI-anchor (GPI-SOM)

hit not hit

Subcellular Localization (HECTAR)

Statistics Setting

Totaling Search Result by

Option

Median Gene Ontology Top 10

Search Hints

Note

The statistical table of checked item is expressed as a search result.
To express the bar chart of checked item, please click the title in column name.
To express the list of extracted IDs, please click the title in line name.
Sometimes, there is a difference in hit counts between KOG and others, because KOG allows duplicated data.

Species	Binomina	Number of non-redundant sequences	Class	Habitat	Unicellular/Multicellular
A. thaliana	Arabidopsis thaliana	26,947	Land plants	Terrestrial	Multicellular
P. patens	Physcomitrella patens	25,775
S. moellendorffii	Selaginella moellendorffii	20,225
K. flaccidum	Klebsormidium flaccidum	15,974	Green algae	Freshwater
B. prasinos	Bathycoccus prasinos	7,734		Marine	Unicellular
M. pusilla	Micromonas pusilla	10,557
M. sp. RCC299	Micromonas sp. RCC299	9,969
O. tauri	Ostreococcus tauri	7,602
O. lucimarinus	Ostreococcus lucimarinus	7,688
C. reinhardtii	Chlamydomonas reinhardtii	15,597		Freshwater
M. neglectum	Monoraphidium neglectum	14,128
V. carteri	Volvox carteri	13,925
A. protothecoides	Auxenochlorella protothecoides	6,628
C. variabilis	Chlorella variabilis	9,783
C. subellipsoidea C-169	Coccomyxa subellipsoidea C-169	9,374
C. crispus	Chondrus crispus	8,331	Red algae	Marine	Multicellular
C. merolae	Cyanidioschyzon merolae	4,642		Freshwater	Unicellular
G. sulphuraria	Galdieria sulphuraria	6,371		Freshwater
P. purpureum	Porphyridium purpureum	8,202		Ubiquitous
P. yezoensis	Pyropia yezoensis	9,731		Marine	Multicellular
C. paradoxa	Cyanophora paradoxa	31,147	Glaucophyceae	Freshwater	Unicellular
P. capsici	Phytophthora capsici	16,168	Oomycetes	Terrestrial
P. infestans	Phytophthora infestans	19,455
P. ramorum	Phytophthora ramorum	13,202
P. sojae	Phytophthora sojae	16,833
F. cylindrus CCMP 1102	Fragilariopsis cylindrus CCMP 1102	20,616	Diatoms	Marine
P. tricornutum	Phaeodactylum tricornutum	10,063
T. pseudonana	Thalassiosira pseudonana	11,484
A. anophagefferens	Aureococcus anophagefferens	10,772	Other microalgae
E. siliculosus	Ectocarpus siliculosus	15,557			Multicellular
S. minutum	Symbiodinium minutum	42,522			Unicellular
E. huxleyi	Emiliania huxleyi	21,992
G. theta	Guillardia theta	19,964
B. natans	Bigelowiella natans	21,145

The sequence sets are nonredundant sets of each whole sequences which executed
by using the CD-HIT tool

with the "sequence identity threshold = 0.9" and the "search word size' = 5" options.

"Length" is protein length.

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	414.2	354.0
P. patens	391.6	325.0
S. moellendorffii	385.6	321.0
K. flaccidum	536.2	422.0
B. prasinos	508.4	415.0
M. pusilla	441.0	351.0
M. sp. RCC299	471.5	370.0
O. tauri	384.7	309.0
O. lucimarinus	380.1	305.0
C. reinhardtii	499.7	346.0
M. neglectum	367.5	287.0
V. carteri	568.8	389.0
A. protothecoides	395.9	321.0
C. variabilis	402.8	316.0
C. subellipsoidea C-169	424.6	335.0
C. crispus	380.1	293.0
C. merolae	504.3	416.0
G. sulphuraria	419.0	349.0
P. purpureum	475.6	389.0
P. yezoensis	292.2	215.0

Species	Average	Median
C. paradoxa	230.2	165.0
P. capsici	388.4	313.0
P. infestans	407.0	306.0
P. ramorum	505.1	402.0
P. sojae	517.1	400.0
F. cylindrus CCMP 1102	429.1	344.0
P. tricornutum	459.7	381.0
T. pseudonana	492.2	391.0
A. anophagefferens	519.0	357.0
E. siliculosus	519.1	393.0
S. minutum	555.1	374.0
E. huxleyi	401.0	327.0
G. theta	432.4	327.0
B. natans	436.5	338.0

"Charged" is the rate of charged amino acid residues (DEHKR).

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	26.1%	26.1%
P. patens	24.8%	24.9%
S. moellendorffii	25.5%	25.5%
K. flaccidum	25.9%	26.2%
B. prasinos	30.2%	30.1%
M. pusilla	27.6%	27.6%
M. sp. RCC299	27.4%	27.4%
O. tauri	28.2%	28.2%
O. lucimarinus	28.5%	28.6%
C. reinhardtii	21.9%	21.7%
M. neglectum	22.3%	22.2%
V. carteri	22.7%	22.4%
A. protothecoides	23.6%	23.6%
C. variabilis	24.1%	24.3%
C. subellipsoidea C-169	24.2%	24.4%
C. crispus	25.7%	25.8%
C. merolae	25.4%	25.7%
G. sulphuraria	26.1%	26.2%
P. purpureum	26.0%	26.1%
P. yezoensis	23.0%	23.1%

Species	Average	Median
C. paradoxa	26.0%	25.9%
P. capsici	26.1%	26.2%
P. infestans	26.4%	26.6%
P. ramorum	26.0%	26.2%
P. sojae	26.4%	26.6%
F. cylindrus CCMP 1102	25.8%	26.0%
P. tricornutum	25.2%	25.4%
T. pseudonana	25.9%	26.0%
A. anophagefferens	26.2%	26.1%
E. siliculosus	25.8%	25.8%
S. minutum	26.4%	26.1%
E. huxleyi	24.0%	24.0%
G. theta	27.7%	27.7%
B. natans	28.1%	28.1%

"Nonpolar" is the rate of nonpolar amino acid residues (ACFGILMPVWY).

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	48.0%	47.8%
P. patens	46.3%	46.2%
S. moellendorffii	45.5%	45.3%
K. flaccidum	45.2%	45.2%
B. prasinos	51.7%	51.6%
M. pusilla	44.3%	44.2%
M. sp. RCC299	44.3%	44.3%
O. tauri	46.3%	46.3%
O. lucimarinus	46.2%	46.3%
C. reinhardtii	40.5%	40.2%
M. neglectum	40.0%	39.8%
V. carteri	42.2%	42.1%
A. protothecoides	41.2%	41.1%
C. variabilis	43.6%	43.7%
C. subellipsoidea C-169	43.7%	43.8%
C. crispus	46.2%	46.3%
C. merolae	45.5%	45.4%
G. sulphuraria	48.5%	48.2%
P. purpureum	45.6%	45.6%
P. yezoensis	39.8%	40.0%

Species	Average	Median
C. paradoxa	42.9%	42.8%
P. capsici	47.4%	47.3%
P. infestans	47.8%	47.7%
P. ramorum	47.3%	47.3%
P. sojae	47.3%	47.3%
F. cylindrus CCMP 1102	51.2%	51.1%
P. tricornutum	47.1%	47.1%
T. pseudonana	48.8%	48.9%
A. anophagefferens	41.2%	41.1%
E. siliculosus	44.9%	44.8%
S. minutum	46.2%	45.9%
E. huxleyi	40.9%	40.9%
G. theta	48.6%	48.3%
B. natans	49.4%	49.3%

"Acidic" is the rate of acidic amino acid residues (DE).

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	11.8%	11.7%
P. patens	10.8%	11.0%
S. moellendorffii	11.5%	11.4%
K. flaccidum	12.0%	12.0%
B. prasinos	14.3%	14.1%
M. pusilla	12.9%	12.8%
M. sp. RCC299	12.9%	12.8%
O. tauri	13.0%	13.2%
O. lucimarinus	13.5%	13.5%
C. reinhardtii	9.5%	9.4%
M. neglectum	10.2%	10.0%
V. carteri	10.0%	9.8%
A. protothecoides	10.5%	10.6%
C. variabilis	11.1%	11.2%
C. subellipsoidea C-169	11.1%	11.2%
C. crispus	10.3%	10.8%
C. merolae	11.0%	11.1%
G. sulphuraria	11.7%	11.8%
P. purpureum	12.1%	12.0%
P. yezoensis	10.6%	10.5%

Species	Average	Median
C. paradoxa	11.7%	11.5%
P. capsici	12.3%	12.1%
P. infestans	11.8%	11.7%
P. ramorum	12.4%	12.3%
P. sojae	12.3%	12.3%
F. cylindrus CCMP 1102	12.7%	12.6%
P. tricornutum	11.9%	11.8%
T. pseudonana	12.7%	12.7%
A. anophagefferens	12.2%	12.2%
E. siliculosus	12.2%	12.1%
S. minutum	12.4%	12.1%
E. huxleyi	10.9%	10.8%
G. theta	13.1%	12.8%
B. natans	12.2%	12.0%

"Basic" is the rate of basic amino acid residues (HKR).

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	14.2%	14.1%
P. patens	13.9%	13.7%
S. moellendorffii	14.0%	13.8%
K. flaccidum	13.9%	13.9%
B. prasinos	15.9%	15.7%
M. pusilla	14.8%	14.4%
M. sp. RCC299	14.5%	14.2%
O. tauri	15.2%	14.8%
O. lucimarinus	15.0%	14.6%
C. reinhardtii	12.4%	12.0%
M. neglectum	12.1%	11.9%
V. carteri	12.7%	12.4%
A. protothecoides	13.1%	12.7%
C. variabilis	13.0%	12.9%
C. subellipsoidea C-169	13.1%	13.0%
C. crispus	15.3%	14.9%
C. merolae	14.5%	14.2%
G. sulphuraria	14.4%	14.2%
P. purpureum	13.9%	13.8%
P. yezoensis	12.4%	12.3%

Species	Average	Median
C. paradoxa	14.3%	13.8%
P. capsici	13.8%	13.8%
P. infestans	14.6%	14.5%
P. ramorum	13.6%	13.7%
P. sojae	14.0%	14.0%
F. cylindrus CCMP 1102	13.1%	13.1%
P. tricornutum	13.3%	13.3%
T. pseudonana	13.2%	13.1%
A. anophagefferens	14%	13.7%
E. siliculosus	13.6%	13.4%
S. minutum	14.0%	13.8%
E. huxleyi	13.1%	12.9%
G. theta	14.6%	14.5%
B. natans	15.9%	15.8%

"GRAVY" is a value of grand average of hydropathicity index.
go to GRAVY calculation page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	-0.30	-0.30
P. patens	-0.24	-0.25
S. moellendorffii	-0.22	-0.21
K. flaccidum	-0.32	-0.33
B. prasinos	-0.53	-0.54
M. pusilla	-0.32	-0.32
M. sp. RCC299	-0.32	-0.32
O. tauri	-0.32	-0.32
O. lucimarinus	-0.32	-0.32
C. reinhardtii	-0.16	-0.16
M. neglectum	-0.17	-0.16
V. carteri	-0.20	-0.21
A. protothecoides	-0.14	-0.15
C. variabilis	-0.21	-0.23
C. subellipsoidea C-169	-0.22	-0.23
C. crispus	-0.29	-0.30
C. merolae	-0.25	-0.27
G. sulphuraria	-0.28	-0.28
P. purpureum	-0.24	-0.25
P. yezoensis	-0.04	-0.06

Species	Average	Median
C. paradoxa	-0.27	-0.25
P. capsici	-0.30	-0.31
P. infestans	-0.32	-0.33
P. ramorum	-0.30	-0.31
P. sojae	-0.33	-0.34
F. cylindrus CCMP 1102	-0.44	-0.46
P. tricornutum	-0.28	-0.30
T. pseudonana	-0.36	-0.38
A. anophagefferens	-0.17	-0.17
E. siliculosus	-0.33	-0.34
S. minutum	-0.31	-0.31
E. huxleyi	-0.16	-0.17
G. theta	-0.39	-0.39
B. natans	-0.43	-0.44

"pI" is a isoelectric point.

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	7.3	7.2
P. patens	7.5	7.4
S. moellendorffii	7.3	7.0
K. flaccidum	7.2	6.7
B. prasinos	7.1	6.4
M. pusilla	7.2	6.4
M. sp. RCC299	7.0	6.2
O. tauri	7.3	6.5
O. lucimarinus	6.9	6.1
C. reinhardtii	7.6	7.4
M. neglectum	7.4	7.1
V. carteri	7.5	7.4
A. protothecoides	7.3	6.6
C. variabilis	7.0	6.4
C. subellipsoidea C-169	7.1	6.5
C. crispus	8.3	8.6
C. merolae	7.9	8.0
G. sulphuraria	7.4	7.2
P. purpureum	7.0	6.5
P. yezoensis	7.3	6.7

Species	Average	Median
C. paradoxa	7.6	7.3
P. capsici	6.9	6.3
P. infestans	7.4	7.2
P. ramorum	6.7	6.1
P. sojae	6.9	6.3
F. cylindrus CCMP 1102	6.5	5.8
P. tricornutum	6.8	6.2
T. pseudonana	6.3	5.7
A. anophagefferens	7.0	6.4
E. siliculosus	7.0	6.3
S. minutum	6.9	6.3
E. huxleyi	7.3	6.9
G. theta	6.9	6.3
B. natans	8.0	8.6

"Solubility" was calculated with the SOLpro prediction tool for detecting protein solubility in E. coli.
The soluble probability value is expressed more than 0.5.
go to SOLpro page

Alga-PrAS (all data) –soluble/insoluble protein %

Species	soluble	insoluble
A. thaliana	37.4%	62.6%
P. patens	41.9%	58.1%
S. moellendorffii	36.0%	64.0%
K. flaccidum	51.2%	48.8%
B. prasinos	36.8%	63.2%
M. pusilla	53.3%	46.7%
M. sp. RCC299	52.1%	47.9%
O. tauri	43.8%	56.2%
O. lucimarinus	46.4%	53.6%
C. reinhardtii	51.4%	48.6%
M. neglectum	54.3%	45.7%
V. carteri	46.4%	53.6%
A. protothecoides	50.6%	49.4%
C. variabilis	47.0%	53.0%
C. subellipsoidea C-169	46.3%	53.7%
C. crispus	39.0%	61.0%
C. merolae	22.0%	78.0%
G. sulphuraria	31.5%	68.5%
P. purpureum	28.5%	71.5%
P. yezoensis	47.9%	52.1%

Species	soluble	insoluble
C. paradoxa	56.2%	43.8%
P. capsici	37.7%	62.3%
P. infestans	32.3%	67.7%
P. ramorum	33.7%	66.3%
P. sojae	35.3%	64.7%
F. cylindrus CCMP 1102	31.7%	68.3%
P. tricornutum	27.7%	72.3%
T. pseudonana	30.8%	69.2%
A. anophagefferens	51.5%	48.5%
E. siliculosus	50.5%	49.5%
S. minutum	44.7%	55.3%
E. huxleyi	42.4%	57.6%
G. theta	38.3%	61.7%
B. natans	35.3%	64.7%

"β Sheet" is the rate of β sheet of protein secondary structure calculated with the SSpro prediction tool.
go to SSpro page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	13.8%	12.3%
P. patens	13.4%	12.4%
S. moellendorffii	13.3%	12.3%
K. flaccidum	11.9%	11.0%
B. prasinos	12.2%	11.5%
M. pusilla	11.7%	10.6%
M. sp. RCC299	12.2%	11.2%
O. tauri	12.9%	12.0%
O. lucimarinus	12.9%	11.9%
C. reinhardtii	10.8%	9.2%
M. neglectum	10.4%	8.8%
V. carteri	11.7%	10.4%
A. protothecoides	12.1%	11.1%
C. variabilis	12.1%	11.0%
C. subellipsoidea C-169	12.2%	11.2%
C. crispus	13.3%	12.2%
C. merolae	12.3%	11.5%
G. sulphuraria	13.7%	12.8%
P. purpureum	12.3%	11.4%
P. yezoensis	12.9%	11.8%

Species	Average	Median
C. paradoxa	11.3%	9.5%
P. capsici	12.7%	11.8%
P. infestans	12.8%	11.8%
P. ramorum	12.3%	11.3%
P. sojae	11.9%	11.1%
F. cylindrus CCMP 1102	13.3%	12.8%
P. tricornutum	12.7%	11.9%
T. pseudonana	12.8%	12.0%
A. anophagefferens	12.7%	11.6%
E. siliculosus	11.6%	10.3%
S. minutum	11.0%	10.2%
E. huxleyi	11.0%	10.2%
G. theta	11.9%	10.7%
B. natans	12.7%	11.8%

"Disorder" is the rate of protein disordered region calculated with the DISOPRED3 prediction tool.
go to DISOPRED3 page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	22.5%	15.6%
P. patens	19.5%	12.9%
S. moellendorffii	11.8%	6.6%
K. flaccidum	20.1%	13.8%
B. prasinos	21.9%	18.1%
M. pusilla	19.9%	15.4%
M. sp. RCC299	18.5%	13.6%
O. tauri	14.3%	10.1%
O. lucimarinus	13.8%	9.1%
C. reinhardtii	21.4%	15.1%
M. neglectum	19.7%	14.6%
V. carteri	21.0%	14.4%
A. protothecoides	15.1%	10.8%
C. variabilis	16.4%	10.3%
C. subellipsoidea C-169	16.7%	10.5%
C. crispus	14.1%	9.2%
C. merolae	17.5%	13.5%
G. sulphuraria	13.9%	9.3%
P. purpureum	16.4%	12.2%
P. yezoensis	11.7%	7.8%

Species	Average	Median
C. paradoxa	13.8%	8.3%
P. capsici	16.0%	10.5%
P. infestans	15.6%	10.3%
P. ramorum	19.8%	14.2%
P. sojae	20.7%	15.0%
F. cylindrus CCMP 1102	17.5%	12.4%
P. tricornutum	15.2%	10.3%
T. pseudonana	17.7%	11.7%
A. anophagefferens	12.1%	7.4%
E. siliculosus	21.5%	15.7%
S. minutum	13.6%	8.6%
E. huxleyi	15.0%	10.8%
G. theta	15.5%	10.2%
B. natans	15.7%	10.9%

"Long Disorder" was calculated with the DISOPRED3 prediction tool
for detecting protein long disordered region (>30 consecutive regidues).
go to DISOPRED3 page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	0.88	1.00
P. patens	0.78	0
S. moellendorffii	0.43	0
K. flaccidum	1.01	0
B. prasinos	1.11	1.00
M. pusilla	0.88	0
M. sp. RCC299	0.88	0
O. tauri	0.52	0
O. lucimarinus	0.49	0
C. reinhardtii	1.12	0
M. neglectum	0.72	0
V. carteri	1.24	0
A. protothecoides	0.57	0
C. variabilis	0.64	0
C. subellipsoidea C-169	0.69	0
C. crispus	0.51	0
C. merolae	0.83	0
G. sulphuraria	0.56	0
P. purpureum	0.76	0
P. yezoensis	0.31	0

Species	Average	Median
C. paradoxa	0.23	0
P. capsici	0.58	0
P. infestans	0.65	0
P. ramorum	0.96	1.00
P. sojae	1.03	1.00
F. cylindrus CCMP 1102	0.69	0
P. tricornutum	0.67	0
T. pseudonana	0.87	0
A. anophagefferens	0.67	0
E. siliculosus	1.14	0
S. minutum	0.64	0
E. huxleyi	0.52	0
G. theta	0.58	0
B. natans	0.65	0

"Signal" was calculated with the SignalP prediction tool for a detecting signal peptide cleavage site.
go to SignalP page

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	13.1%	86.9%
P. patens	6.9%	93.1%
S. moellendorffii	7.9%	92.1%
K. flaccidum	7.2%	92.8%
B. prasinos	4.3%	95.7%
M. pusilla	4.1%	95.9%
M. sp. RCC299	4.6%	95.4%
O. tauri	3.1%	96.9%
O. lucimarinus	3.1%	96.9%
C. reinhardtii	7.4%	92.6%
M. neglectum	6.9%	93.1%
V. carteri	7.7%	92.3%
A. protothecoides	5.2%	94.8%
C. variabilis	7.8%	92.2%
C. subellipsoidea C-169	7.8%	92.2%
C. crispus	4.7%	95.3%
C. merolae	3.7%	96.3%
G. sulphuraria	4.0%	96.0%
P. purpureum	4.5%	95.5%
P. yezoensis	4.6%	95.4%

Species	hit	not hit
C. paradoxa	3.5%	96.5%
P. capsici	9.3%	90.7%
P. infestans	7.5%	92.5%
P. ramorum	12.2%	87.8%
P. sojae	11.9%	88.1%
F. cylindrus CCMP 1102	10.7%	89.3%
P. tricornutum	13.8%	86.2%
T. pseudonana	13.1%	86.9%
A. anophagefferens	12.9%	87.1%
E. siliculosus	9.7%	90.3%
S. minutum	7.6%	92.4%
E. huxleyi	12.5%	87.5%
G. theta	12.8%	87.2%
B. natans	9.9%	90.1%

"Membrane" was calculated with the TMHMM prediction tool for detecting transmembrane helices in protein.
go to TMHMM page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	0.89	0
P. patens	0.79	0
S. moellendorffii	0.62	0
K. flaccidum	0.63	0
B. prasinos	0.69	0
M. pusilla	0.58	0
M. sp. RCC299	0.68	0
O. tauri	0.66	0
O. lucimarinus	0.72	0
C. reinhardtii	0.58	0
M. neglectum	0.67	0
V. carteri	0.55	0
A. protothecoides	0.71	0
C. variabilis	0.75	0
C. subellipsoidea C-169	0.78	0
C. crispus	0.52	0
C. merolae	0.70	0
G. sulphuraria	0.91	0
P. purpureum	0.71	0
P. yezoensis	0.63	0

Species	Average	Median
C. paradoxa	0.56	0
P. capsici	0.74	0
P. infestans	0.61	0
P. ramorum	0.77	0
P. sojae	0.69	0
F. cylindrus CCMP 1102	0.77	0
P. tricornutum	0.81	0
T. pseudonana	0.65	0
A. anophagefferens	0.58	0
E. siliculosus	0.60	0
S. minutum	0.66	0
E. huxleyi	0.68	0
G. theta	0.64	0
B. natans	0.71	0

"N-terminal Topology", such as intracellular or extracellular in N-terminal position, was calculated with the TMHMM prediction tool for detecting transmembrane helices and topolgoy in protein.
go to TMHMM page

Alga-PrAS (all data) – number of hits/400aa

Species	intracellular	extracellular
A. thaliana	17.9%	82.1%
P. patens	15.9%	84.1%
S. moellendorffii	11.0%	89.0%
K. flaccidum	12.3%	87.7%
B. prasinos	16.9%	83.1%
M. pusilla	11.4%	88.6%
M. sp. RCC299	12.1%	87.9%
O. tauri	13.3%	86.7%
O. lucimarinus	13.4%	86.6%
C. reinhardtii	11.9%	88.1%
M. neglectum	11.5%	88.5%
V. carteri	11.2%	88.8%
A. protothecoides	11.5%	88.5%
C. variabilis	12.2%	87.8%
C. subellipsoidea C-169	13.2%	86.8%
C. crispus	13.0%	87.0%
C. merolae	12.3%	87.7%
G. sulphuraria	15.5%	84.5%
P. purpureum	13.6%	86.4%
P. yezoensis	10.9%	89.1%

Species	intracellular	extracellular
C. paradoxa	12.6%	87.4%
P. capsici	11.8%	88.2%
P. infestans	11.3%	88.7%
P. ramorum	12.0%	88.0%
P. sojae	11.6%	88.4%
F. cylindrus CCMP 1102	17.0%	83.0%
P. tricornutum	16.5%	83.5%
T. pseudonana	13.0%	87.0%
A. anophagefferens	9.6%	90.4%
E. siliculosus	11.5%	88.5%
S. minutum	12.3%	87.7%
E. huxleyi	11.1%	88.9%
G. theta	13.3%	86.7%
B. natans	14.9%	85.1%

"S-S Bond" was calculated with the DIpro prediction tool for detecting a cysteine disulfide bond in protein.
go to DIpro page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	3.13	2.54
P. patens	3.40	2.70
S. moellendorffii	3.24	2.86
K. flaccidum	2.46	2.08
B. prasinos	2.48	2.17
M. pusilla	2.32	1.94
M. sp. RCC299	2.57	2.16
O. tauri	2.58	2.30
O. lucimarinus	2.60	2.25
C. reinhardtii	2.84	2.23
M. neglectum	2.28	1.99
V. carteri	3.04	2.47
A. protothecoides	2.56	2.07
C. variabilis	2.53	2.07
C. subellipsoidea C-169	2.57	2.11
C. crispus	3.31	2.54
C. merolae	2.79	2.54
G. sulphuraria	3.08	2.80
P. purpureum	2.42	2.22
P. yezoensis	2.08	1.71

Species	Average	Median
C. paradoxa	2.15	1.66
P. capsici	2.39	2.07
P. infestans	2.78	2.50
P. ramorum	2.48	2.20
P. sojae	2.53	2.22
F. cylindrus CCMP 1102	2.62	2.26
P. tricornutum	2.47	2.26
T. pseudonana	2.55	2.27
A. anophagefferens	2.90	2.64
E. siliculosus	2.66	2.25
S. minutum	3.31	3.03
E. huxleyi	3.14	2.78
G. theta	2.99	2.64
B. natans	2.68	2.34

"Domain Linker" was calculated with the DROP(DLP-SVM) prediction tool.
go to DROP(DLP-SVM) page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	2.13	1.35
P. patens	1.85	0.93
S. moellendorffii	1.71	0.64
K. flaccidum	1.74	0.87
B. prasinos	1.26	0
M. pusilla	1.83	1.02
M. sp. RCC299	1.83	1.01
O. tauri	1.70	0.70
O. lucimarinus	1.76	0.82
C. reinhardtii	1.84	0.86
M. neglectum	2.02	1.13
V. carteri	1.73	0.83
A. protothecoides	2.21	1.46
C. variabilis	2.02	1.17
C. subellipsoidea C-169	1.98	1.14
C. crispus	1.86	0.78
C. merolae	1.76	0.94
G. sulphuraria	1.58	0.62
P. purpureum	1.52	0.61
P. yezoensis	2.20	1.22

Species	Average	Median
C. paradoxa	2.17	0.88
P. capsici	1.62	0.55
P. infestans	1.49	0
P. ramorum	1.45	0.45
P. sojae	1.45	0.47
F. cylindrus CCMP 1102	1.19	0
P. tricornutum	1.55	0.55
T. pseudonana	1.37	0
A. anophagefferens	1.73	0.79
E. siliculosus	1.54	0.54
S. minutum	1.18	0
E. huxleyi	1.78	0.95
G. theta	1.29	0
B. natans	1.35	0

"Internal Repeat" was calculated with the RADAR prediction tool for detecting internal repeats in protein.
go to RADAR page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	1.66	1.56
P. patens	1.54	1.42
S. moellendorffii	1.57	1.48
K. flaccidum	1.90	1.84
B. prasinos	1.80	1.72
M. pusilla	2.01	1.91
M. sp. RCC299	1.95	1.86
O. tauri	1.82	1.72
O. lucimarinus	1.84	1.75
C. reinhardtii	1.89	1.78
M. neglectum	2.11	2.04
V. carteri	1.89	1.79
A. protothecoides	1.94	1.84
C. variabilis	1.71	1.63
C. subellipsoidea C-169	1.74	1.66
C. crispus	1.61	1.49
C. merolae	1.85	1.80
G. sulphuraria	1.59	1.49
P. purpureum	1.67	1.61
P. yezoensis	1.82	1.73

Species	Average	Median
C. paradoxa	1.75	1.55
P. capsici	1.61	1.53
P. infestans	1.48	1.40
P. ramorum	1.74	1.63
P. sojae	1.81	1.71
F. cylindrus CCMP 1102	1.73	1.65
P. tricornutum	1.62	1.54
T. pseudonana	1.69	1.61
A. anophagefferens	1.94	1.85
E. siliculosus	1.86	1.77
S. minutum	1.72	1.66
E. huxleyi	2.13	2.05
G. theta	1.68	1.60
B. natans	1.6	1.54

"PEST Region" was calculated with the ePESTfind prediction tool
for detecting PEST regions in protein.
go to ePESTfind page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	0.32	0
P. patens	0.27	0
S. moellendorffii	0.20	0
K. flaccidum	0.39	0
B. prasinos	0.34	0
M. pusilla	0.43	0
M. sp. RCC299	0.40	0
O. tauri	0.26	0
O. lucimarinus	0.27	0
C. reinhardtii	0.23	0
M. neglectum	0.19	0
V. carteri	0.26	0
A. protothecoides	0.26	0
C. variabilis	0.27	0
C. subellipsoidea C-169	0.26	0
C. crispus	0.27	0
C. merolae	0.31	0
G. sulphuraria	0.20	0
P. purpureum	0.25	0
P. yezoensis	0.21	0

Species	Average	Median
C. paradoxa	0.32	0
P. capsici	0.37	0
P. infestans	0.32	0
P. ramorum	0.41	0
P. sojae	0.45	0
F. cylindrus CCMP 1102	0.44	0
P. tricornutum	0.36	0
T. pseudonana	0.42	0
A. anophagefferens	0.22	0
E. siliculosus	0.41	0
S. minutum	0.36	0
E. huxleyi	0.29	0
G. theta	0.30	0
B. natans	0.30	0

"Pfam" is the database of a large collection of protein families,which calculated with the HMMPfam tool. Only top hit of Pfam is stored in the database. We use Pfam v28.0 as a reference database.
go to Pfam page

Alga-PrAS (all data)

Species	hit	not hit	DUF
A. thaliana	74.7%	19.8%	5.5%
P. patens	61.0%	35.3%	3.6%
S. moellendorffii	67.8%	29.2%	3.0%
K. flaccidum	61.5%	34.9%	3.6%
B. prasinos	68.4%	27.8%	3.8%
M. pusilla	61.9%	34.8%	3.3%
M. sp. RCC299	66.0%	30.3%	3.7%
O. tauri	65.0%	31.5%	3.5%
O. lucimarinus	68.4%	27.6%	3.9%
C. reinhardtii	50.6%	47.0%	2.4%
M. neglectum	52.6%	45.0%	2.4%
V. carteri	54.3%	43.3%	2.4%
A. protothecoides	67.2%	29.5%	3.3%
C. variabilis	65.0%	32.2%	2.9%
C. subellipsoidea C-169	66.4%	30.5%	3.1%
C. crispus	52.3%	45.8%	1.9%
C. merolae	74.9%	22.0%	3.1%
G. sulphuraria	71.3%	25.9%	2.8%
P. purpureum	66.3%	30.2%	3.5%
P. yezoensis	52.9%	45.1%	2.1%

Species	hit	not hit	DUF
C. paradoxa	31.4%	67.5%	1.1%
P. capsici	58.4%	40.0%	1.6%
P. infestans	52.2%	46.3%	1.5%
P. ramorum	64.6%	33.4%	2.1%
P. sojae	58.6%	39.8%	1.6%
F. cylindrus CCMP 1102	45.8%	51.5%	2.7%
P. tricornutum	62.6%	34.4%	3.0%
T. pseudonana	60.1%	37.3%	2.6%
A. anophagefferens	70.7%	27.3%	2.1%
E. siliculosus	57.4%	40.0%	2.5%
S. minutum	42.4%	56.2%	1.5%
E. huxleyi	52.0%	45.9%	2.1%
G. theta	48.7%	49.4%	1.9%
B. natans	53.5%	44.3%	2.1%

"CDD" (Conserved Domain Database) is a database of well-annotated multiple sequence alignment models and derived database search models, for ancient domains and full-length proteins. We use cdd_v20170328 as a reference database.
go to CDD page

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	75.2%	24.8%
P. patens	61.0%	39.0%
S. moellendorffii	66.9%	33.1%
K. flaccidum	59.1%	40.9%
B. prasinos	62.8%	37.2%
M. pusilla	56.7%	43.3%
M. sp. RCC299	61.8%	38.2%
O. tauri	61.5%	38.5%
O. lucimarinus	64.3%	35.7%
C. reinhardtii	46.2%	53.8%
M. neglectum	49.0%	51.0%
V. carteri	51.0%	49.0%
A. protothecoides	64.8%	35.2%
C. variabilis	62.7%	37.3%
C. subellipsoidea C-169	64.4%	35.6%
C. crispus	50.7%	49.3%
C. merolae	71.5%	28.5%
G. sulphuraria	68.1%	31.9%
P. purpureum	62.9%	37.1%
P. yezoensis	48.8%	51.2%

Species	hit	not hit
C. paradoxa	27.7%	72.3%
P. capsici	52.4%	47.6%
P. infestans	45.6%	54.4%
P. ramorum	60.0%	40.0%
P. sojae	54.2%	45.8%
F. cylindrus CCMP 1102	39.4%	60.6%
P. tricornutum	58.4%	41.6%
T. pseudonana	55.1%	44.9%
A. anophagefferens	65.3%	34.7%
E. siliculosus	52.8%	47.2%
S. minutum	36.1%	63.9%
E. huxleyi	43.6%	56.4%
G. theta	42.2%	57.8%
B. natans	47.1%	52.9%

"UniProtKB/Swiss-Prot" (UniProtKB/Swiss-Prot: approximately 530,000 seqs) is the manually annotated and reviewed section of the UniProt Knowledgebase. Only top hit of UniProtKB/Swiss-Prot is stored in the database. We use UniProtKB/TrEMBL released v2014_04 as a reference database.
more information

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	74.5%	25.5%
P. patens	58.6%	41.4%
S. moellendorffii	64.1%	35.9%
K. flaccidum	52.8%	47.2%
B. prasinos	54.4%	45.6%
M. pusilla	49.7%	50.3%
M. sp. RCC299	54.9%	45.1%
O. tauri	54.4%	45.6%
O. lucimarinus	56.7%	43.3%
C. reinhardtii	40.4%	59.6%
M. neglectum	44.8%	55.2%
V. carteri	44.9%	55.1%
A. protothecoides	60.2%	39.8%
C. variabilis	57.6%	42.4%
C. subellipsoidea C-169	59.3%	40.7%
C. crispus	45.7%	54.3%
C. merolae	63.5%	36.5%
G. sulphuraria	61.8%	38.2%
P. purpureum	53.7%	46.3%
P. yezoensis	39.9%	60.1%

Species	hit	not hit
C. paradoxa	23.4%	76.6%
P. capsici	46.5%	53.5%
P. infestans	36.8%	63.2%
P. ramorum	53.5%	46.5%
P. sojae	47.6%	52.4%
F. cylindrus CCMP 1102	29.6%	70.4%
P. tricornutum	47.4%	52.6%
T. pseudonana	44.3%	55.7%
A. anophagefferens	57.6%	42.4%
E. siliculosus	45.5%	54.5%
S. minutum	27.4%	72.6%
E. huxleyi	33.1%	66.9%
G. theta	34.0%	66.0%
B. natans	38.1%	61.9%

"UniProtKB/TrEMBL" (Eukaryote: approximately 20,000,000 seqs) is the manually annotated and reviewed section of the UniProt Knowledgebase. Only top hit of UniProtKB/TrEMBL (Eukaryote) is stored in the database. Poor annotations, such as "Uncharacterized protein", "Putative uncharacterized", "Unnamed product", and just ID, are excluded. We use UniProtKB/TrEMBL released v2016_06 as a reference database.
more information

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	78.6%	21.4%
P. patens	13.6%	86.4%
S. moellendorffii	12.6%	86.4%
K. flaccidum	23.5%	76.5%
B. prasinos	33.2%	66.8%
M. pusilla	18.1%	81.9%
M. sp. RCC299	22.0%	78.0%
O. tauri	71.6%	28.4%
O. lucimarinus	34.2%	65.8%
C. reinhardtii	26.5%	73.5%
M. neglectum	32.9%	67.1%
V. carteri	13.7%	86.3%
A. protothecoides	63.8%	36.2%
C. variabilis	44.1%	55.9%
C. subellipsoidea C-169	44.9%	55.1%
C. crispus	21.6%	78.4%
C. merolae	59.2%	40.8%
G. sulphuraria	64.1%	35.9%
P. purpureum	34.7%	65.3%
P. yezoensis	29.7%	70.3%

Species	hit	not hit
C. paradoxa	14.3%	85.7%
P. capsici	20.7%	79.3%
P. infestans	34.0%	66.0%
P. ramorum	5.7%	94.3%
P. sojae	12.1%	87.9%
F. cylindrus CCMP 1102	11.0%	89.0%
P. tricornutum	12.2%	87.8%
T. pseudonana	17.4%	82.6%
A. anophagefferens	13.3%	86.7%
E. siliculosus	34.4%	65.6%
S. minutum	16.5%	83.5%
E. huxleyi	8.4%	91.6%
G. theta	5.7%	94.3%
B. natans	20.8%	79.2%

PDB(Protein Data Bank) maintains a centralized PDB archive of macromolecular structures. Only top hit of PDB is stored in the database. We use pdbaa released on 2015.10.14 as a reference database.
go to PDB page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	21.7%	0%
P. patens	18.1%	0%
S. moellendorffii	19.8%	0%
K. flaccidum	16.4%	0%
B. prasinos	17.9%	0%
M. pusilla	16.4%	0%
M. sp. RCC299	17.8%	0%
O. tauri	18.3%	0%
O. lucimarinus	18.9%	0%
C. reinhardtii	13.6%	0%
M. neglectum	15.1%	0%
V. carteri	15.0%	0%
A. protothecoides	20.1%	0%
C. variabilis	19.1%	0%
C. subellipsoidea C-169	19.6%	0%
C. crispus	15.7%	0%
C. merolae	22.4%	26.0%
G. sulphuraria	21.8%	24.0%
P. purpureum	17.6%	0%
P. yezoensis	15.2%	0%

Species	Average	Median
C. paradoxa	8.1%	0%
P. capsici	16.1%	0%
P. infestans	11.8%	0%
P. ramorum	17.1%	0%
P. sojae	15.0%	0%
F. cylindrus CCMP 1102	10.2%	0%
P. tricornutum	16.1%	0%
T. pseudonana	15.2%	0%
A. anophagefferens	20.8%	26.0%
E. siliculosus	15.3%	0%
S. minutum	9.8%	0%
E. huxleyi	11.5%	0%
G. theta	11.4%	0%
B. natans	13.4%	0%

"KOG" (EuKaryotic Orthologous Groups) is a eukaryote-specific version of COG (The Clusters of Orthologous Groups) database for identifying ortholog and paralog proteins. Only top hit of KOG is stored in the database. We use KOG version 2015.Apr.25 as a reference database.
go to KOG page

Alga-PrAS (all data)

Species	hit	not hit
Species	✓information storage & processing ✓cellular processes & signaling ✓metabolism ✓poorly characterized	not hit
A. thaliana	45.4%	54.6%
P. patens	39.2%	60.8%
S. moellendorffii	39.9%	60.1%
K. flaccidum	37.5%	62.5%
B. prasinos	42.4%	57.6%
M. pusilla	38.3%	61.7%
M. sp. RCC299	42.0%	58.0%
O. tauri	43.1%	56.9%
O. lucimarinus	44.1%	55.9%
C. reinhardtii	30.9%	69.1%
M. neglectum	32.7%	67.3%
V. carteri	33.9%	66.1%
A. protothecoides	49.7%	50.3%
C. variabilis	44.7%	55.3%
C. subellipsoidea C-169	45.9%	54.1%
C. crispus	36.2%	63.8%
C. merolae	52.8%	47.2%
G. sulphuraria	51.0%	49.0%
P. purpureum	42.9%	57.1%
P. yezoensis	31.1%	68.9%

Species	hit	not hit
Species	✓information storage & processing ✓cellular processes & signaling ✓metabolism ✓poorly characterized	not hit
C. paradoxa	17.2%	82.8%
P. capsici	36.9%	63.1%
P. infestans	30.0%	70.0%
P. ramorum	41.2%	58.8%
P. sojae	33.4%	66.6%
F. cylindrus CCMP 1102	23.2%	76.8%
P. tricornutum	38.3%	61.7%
T. pseudonana	34.9%	65.1%
A. anophagefferens	47.7%	52.3%
E. siliculosus	36.5%	63.5%
S. minutum	19.9%	80.1%
E. huxleyi	25.3%	74.7%
G. theta	26.7%	73.3%
B. natans	30.7%	69.3%

"Gene Ontology" was calculated with the prediction tool of InterProScan for GO annotation. "Gene Ontology" was calculated with the prediction tool of InterProScan 5.15-54.0 for GO annotation.
go to InterProScan page

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	50.8%	49.2%
P. patens	40.9%	59.1%
S. moellendorffii	42.0%	58.0%
K. flaccidum	39.1%	60.9%
B. prasinos	43.3%	56.7%
M. pusilla	38.4%	61.6%
M. sp. RCC299	42.0%	58.0%
O. tauri	42.1%	57.9%
O. lucimarinus	43.7%	56.3%
C. reinhardtii	32.1%	67.9%
M. neglectum	34.0%	66.0%
V. carteri	34.4%	65.6%
A. protothecoides	44.5%	55.5%
C. variabilis	42.6%	57.4%
C. subellipsoidea C-169	43.9%	56.1%
C. crispus	36.2%	63.8%
C. merolae	50.0%	50.0%
G. sulphuraria	47.9%	52.1%
P. purpureum	43.3%	56.7%
P. yezoensis	34.7%	65.3%

Species	hit	not hit
C. paradoxa	19.5%	80.5%
P. capsici	39.1%	60.9%
P. infestans	32.8%	67.2%
P. ramorum	42.8%	57.2%
P. sojae	38.3%	61.7%
F. cylindrus CCMP 1102	28.2%	71.8%
P. tricornutum	39.7%	60.3%
T. pseudonana	38.2%	61.8%
A. anophagefferens	44.7%	55.3%
E. siliculosus	35.6%	64.4%
S. minutum	26.0%	74.0%
E. huxleyi	31.4%	68.6%
G. theta	29.2%	70.8%
B. natans	33.3%	66.7%

"STY-Phosphorylation Site" was calculated with the Musite prediction tool.
go to Musite page

Alga-PrAS (all data)

Species	Average	Median
A. thaliana	2.47	0.95
P. patens	2.55	0.85
S. moellendorffii	1.58	0
K. flaccidum	3.51	1.93
B. prasinos	2.96	2.06
M. pusilla	3.14	1.46
M. sp. RCC299	3.39	1.95
O. tauri	3.05	1.32
O. lucimarinus	2.82	1.22
C. reinhardtii	3.20	1.85
M. neglectum	2.83	1.66
V. carteri	3.07	1.83
A. protothecoides	2.67	1.23
C. variabilis	2.44	0.81
C. subellipsoidea C-169	2.39	0.81
C. crispus	3.08	1.31
C. merolae	2.57	1.45
G. sulphuraria	1.59	0
P. purpureum	2.36	1.11
P. yezoensis	2.36	0.81

Species	Average	Median
C. paradoxa	3.24	0.66
P. capsici	2.15	0
P. infestans	2.39	0.92
P. ramorum	2.94	1.41
P. sojae	3.26	1.68
F. cylindrus CCMP 1102	1.75	0
P. tricornutum	2.31	1.02
T. pseudonana	2.77	1.43
A. anophagefferens	2.13	1.00
E. siliculosus	4.10	2.59
S. minutum	3.01	1.26
E. huxleyi	3.02	1.81
G. theta	2.54	1.20
B. natans	2.51	1.34

"Ubiquitination Site" was calculated with the UbPred prediction tool.
go to UbPred page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	1.36	0
P. patens	1.06	0
S. moellendorffii	0.77	0
K. flaccidum	1.25	0.20
B. prasinos	1.99	1.26
M. pusilla	0.82	0
M. sp. RCC299	0.91	0
O. tauri	0.68	0
O. lucimarinus	0.77	0
C. reinhardtii	0.56	0
M. neglectum	0.47	0
V. carteri	0.62	0
A. protothecoides	0.50	0
C. variabilis	0.87	0
C. subellipsoidea C-169	0.85	0
C. crispus	0.80	0
C. merolae	0.60	0
G. sulphuraria	1.18	0
P. purpureum	0.90	0
P. yezoensis	0.37	0

Species	Average	Median
C. paradoxa	0.80	0
P. capsici	0.93	0
P. infestans	0.90	0
P. ramorum	1.08	0
P. sojae	1.08	0
F. cylindrus CCMP 1102	1.46	0.63
P. tricornutum	0.98	0
T. pseudonana	1.35	0.39
A. anophagefferens	0.46	0
E. siliculosus	1.01	0
S. minutum	1.21	0
E. huxleyi	0.51	0
G. theta	1.55	0
B. natans	1.67	0.77

"N-glycosylation sites" was calculated by combining the results from extracellular location with the TMHMM tool and the sites with the NetNglyc tool. The reason of combining is due to removal of false positive data with NetNglyc.
go to NetNGlyc page

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	0.28	0
P. patens	0.12	0
S. moellendorffii	0.16	0
K. flaccidum	0.18	0
B. prasinos	0.06	0
M. pusilla	0.03	0
M. sp. RCC299	0.05	0
O. tauri	0.03	0
O. lucimarinus	0.03	0
C. reinhardtii	0.12	0
M. neglectum	0.09	0
V. carteri	0.13	0
A. protothecoides	0.07	0
C. variabilis	0.13	0
C. subellipsoidea C-169	0.13	0
C. crispus	0.06	0
C. merolae	0.07	0
G. sulphuraria	0.01	0
P. purpureum	0.06	0
P. yezoensis	0.04	0

Species	Average	Median
C. paradoxa	0.05	0
P. capsici	0.16	0
P. infestans	0.11	0
P. ramorum	0.21	0
P. sojae	0.21	0
F. cylindrus CCMP 1102	0.17	0
P. tricornutum	0.17	0
T. pseudonana	0.18	0
A. anophagefferens	0.13	0
E. siliculosus	0.07	0
S. minutum	0.10	0
E. huxleyi	0.10	0
G. theta	0.18	0
B. natans	0.18	0

"O-Glycosylation Site" was calculated with the plant O-glycosylation motif
"[Ala/Ser/Thr/Val]-Pro_(1-4)-X_(0-10)-[Ala/Ser/Thr/Val]-Pro_(1-4)".
The O-Glycosylation occurs on the hydroxyproline residue(s) of the motif.
go to the reference page of plant O-glycosylation

Alga-PrAS (all data) – number of hits/400aa

Species	Average	Median
A. thaliana	0.80	0
P. patens	0.99	0
S. moellendorffii	0.77	0
K. flaccidum	1.33	0.96
B. prasinos	0.57	0
M. pusilla	1.45	1.10
M. sp. RCC299	1.30	0.99
O. tauri	0.95	0
O. lucimarinus	0.86	0
C. reinhardtii	1.72	1.29
M. neglectum	1.64	1.24
V. carteri	1.52	1.17
A. protothecoides	1.54	1.15
C. variabilis	1.23	0.70
C. subellipsoidea C-169	1.23	0.74
C. crispus	1.33	0.59
C. merolae	1.13	0.91
G. sulphuraria	0.49	0
P. purpureum	0.95	0.68
P. yezoensis	1.60	1.06

Species	Average	Median
C. paradoxa	1.71	0
P. capsici	0.93	0
P. infestans	0.88	0
P. ramorum	1.09	0.72
P. sojae	1.19	0.81
F. cylindrus CCMP 1102	0.73	0
P. tricornutum	0.99	0.60
T. pseudonana	0.89	0.48
A. anophagefferens	1.43	1.10
E. siliculosus	1.23	0.85
S. minutum	1.07	0.58
E. huxleyi	1.60	1.28
G. theta	0.76	0
B. natans	0.71	0

"WoLF PSORT" is the detecting tool of subcellular localization for fungi, animals and plants. We set "plants" as runtime option.
NOTE: The prediction of subcellular localization by WoLF-PSORT might be not appropriate for diatoms (Fragilariopsis cylindrus CCMP 1102, Phaeodactylum tricornutum, and Thalassiosira pseudonana), cryptophytes (Guillardia theta), and dinoflagellates (Symbiodinium minutum) (Gruber A et al., 2015

).
go to WoLF PSORT page

Abbrev	Localization Site	frequency
chlo	chloroplast	34.57%
chlo_mito	chloroplast / mitochondria	0.10%
cysk	cytoskeleton	0.49%
cysk_plas	cytoskeleton / plasma membrane	0.0004%
cyto	cytosol	22.14%
cyto_E.R.	cytosol / endoplasmic reticulum	0.01%
cyto_nucl	cytosol / nuclear	0.33%
cyto_plas	cytosol / plasma membrane	0.01%
E.R.	endoplasmic reticulum	1.00%
E.R._plas	endoplasmic reticulum / plasma membrane	0.05%
E.R._vacu	endoplasmic reticulum / vacuolar membrane	0.001%
extr	extracellular	2.48%
golg	golgi apparatus	0.17%
golg_plas	golgi apparatus / plasma membrane	0.02%
mito	mitochondria	6.31%
mito_plas	mitochondria / plasma membrane	0.002%
nucl	nuclear	23.67%
nucl_plas	nuclear / plasma membrane	0.13%
pero	peroxisome	0.34%
plas	plasma membrane	6.50%
vacu	vacuolar membrane	1.66%

"GPI-anchor" was calculated with the GPI-SOM prediction tool for detecting GPI-anchor signals.
go to GPI-SOM page

Alga-PrAS (all data)

Species	hit	not hit
A. thaliana	2.8%	97.2%
P. patens	2.4%	97.6%
S. moellendorffii	2.3%	97.7%
K. flaccidum	1.9%	98.1%
B. prasinos	1.2%	98.8%
M. pusilla	2.4%	97.6%
M. sp. RCC299	2.5%	97.5%
O. tauri	1.6%	98.4%
O. lucimarinus	1.8%	98.2%
C. reinhardtii	3.5%	96.5%
M. neglectum	4.1%	95.9%
V. carteri	3.0%	97.0%
A. protothecoides	2.6%	97.4%
C. variabilis	2.4%	97.6%
C. subellipsoidea C-169	2.4%	97.6%
C. crispus	1.4%	98.6%
C. merolae	1.4%	98.6%
G. sulphuraria	0.9%	99.1%
P. purpureum	1.9%	98.1%
P. yezoensis	4.1%	95.9%

Species	hit	not hit
C. paradoxa	2.3%	97.7%
P. capsici	2.1%	97.9%
P. infestans	1.6%	98.4%
P. ramorum	2.7%	97.3%
P. sojae	2.8%	97.2%
F. cylindrus CCMP 1102	2.7%	97.3%
P. tricornutum	3.2%	96.8%
T. pseudonana	2.8%	97.2%
A. anophagefferens	3.8%	96.2%
E. siliculosus	2.5%	97.5%
S. minutum	1.8%	98.2%
E. huxleyi	4.0%	96.0%
G. theta	2.0%	98.0%
B. natans	1.4%	98.6%

HECTAR, a subcellular targeting prediction method which takes into account the specific properties of heterokont proteins. The chloroplast transit in diatoms, cryptophytes, and dinoflagellates uses specific motif (ASAFAP motif) (Gruber A et al., 2015

>). HECTAR is the prediction tool of considering this motif. Therefore, we use this tool for diatoms (Fragilariopsis cylindrus CCMP 1102, Phaeodactylum tricornutum, and Thalassiosira pseudonana), cryptophytes (Guillardia theta), and dinoflagellates (Symbiodinium minutum). For the other species in Alga-PrAS, For the HECTAR results were not provided.
go to HECTAR page

Localization Site	frequency
signal peptide	11.97%
signal anchor	3.60%
mitochondrion	2.70%
chloroplast	1.91%
no signal peptide or anchor	0.33%
other localisation	79.48%

Species	Binomina
A. thaliana	Arabidopsis thaliana
P. patens	Physcomitrella patens
S. moellendorffii	Selaginella moellendorffii
K. flaccidum	Klebsormidium flaccidum
B. prasinos	Bathycoccus prasinos
M. pusilla	Micromonas pusilla
M. sp. RCC299	Micromonas sp. RCC299
O. tauri	Ostreococcus tauri
O. lucimarinus	Ostreococcus lucimarinus
C. reinhardtii	Chlamydomonas reinhardtii
M. neglectum	Monoraphidium neglectum
V. carteri	Volvox carteri
A. protothecoides	Auxenochlorella protothecoides
C. variabilis	Chlorella variabilis
C. subellipsoidea C-169	Coccomyxa subellipsoidea C-169
C. crispus	Chondrus crispus
C. merolae	Cyanidioschyzon merolae
G. sulphuraria	Galdieria sulphuraria
P. purpureum	Porphyridium purpureum
P. yezoensis	Pyropia yezoensis
C. paradoxa	Cyanophora paradoxa
P. capsici	Phytophthora capsici
P. infestans	Phytophthora infestans
P. ramorum	Phytophthora ramorum
P. sojae	Phytophthora sojae
F. cylindrus CCMP 1102	Fragilariopsis cylindrus CCMP 1102
P. tricornutum	Phaeodactylum tricornutum
T. pseudonana	Thalassiosira pseudonana
A. anophagefferens	Aureococcus anophagefferens
E. siliculosus	Ectocarpus siliculosus
S. minutum	Symbiodinium minutum
E. huxleyi	Emiliania huxleyi
G. theta	Guillardia theta
B. natans	Bigelowiella natans

Species	Class
A. thaliana	Land plants
P. patens
S. moellendorffii
K. flaccidum	Green algae
B. prasinos
M. pusilla
M. sp. RCC299
O. tauri
O. lucimarinus
C. reinhardtii
M. neglectum
V. carteri
A. protothecoides
C. variabilis
C. subellipsoidea C-169
C. crispus	Red algae
C. merolae
G. sulphuraria
P. purpureum
P. yezoensis
C. paradoxa	Glaucophyceae
P. capsici	Oomycetes
P. infestans
P. ramorum
P. sojae
F. cylindrus CCMP 1102	Diatoms
P. tricornutum
T. pseudonana
A. anophagefferens	Other microalgae
E. siliculosus
S. minutum
E. huxleyi
G. theta
B. natans

Species	Habitat
A. thaliana	Terrestrial
P. patens
S. moellendorffii
K. flaccidum	Freshwater
B. prasinos	Marine
M. pusilla
M. sp. RCC299
O. tauri
O. lucimarinus
C. reinhardtii	Freshwater
M. neglectum
V. carteri
A. protothecoides
C. variabilis
C. subellipsoidea C-169
C. crispus	Marine
C. merolae	Freshwater
G. sulphuraria	Freshwater
P. purpureum	Ubiquitous
P. yezoensis	Marine
C. paradoxa	Freshwater
P. capsici	Terrestrial
P. infestans
P. ramorum
P. sojae
F. cylindrus CCMP 1102	Marine
P. tricornutum
T. pseudonana
A. anophagefferens
E. siliculosus
S. minutum
E. huxleyi
G. theta
B. natans

Species	Unicellular/Multicellular
A. thaliana	Multicellular
P. patens
S. moellendorffii
K. flaccidum
B. prasinos	Unicellular
M. pusilla
M. sp. RCC299
O. tauri
O. lucimarinus
C. reinhardtii
M. neglectum
V. carteri
A. protothecoides
C. variabilis
C. subellipsoidea C-169
C. crispus	Multicellular
C. merolae	Unicellular
G. sulphuraria
P. purpureum
P. yezoensis	Multicellular
C. paradoxa	Unicellular
P. capsici
P. infestans
P. ramorum
P. sojae
F. cylindrus CCMP 1102
P. tricornutum
T. pseudonana
A. anophagefferens
E. siliculosus	Multicellular
S. minutum	Unicellular
E. huxleyi
G. theta
B. natans

KOG	Function	Group
Translation, ribo.	Translation, ribosomal structure and biogenesis	INFORMATION STORAGE AND PROCESSING
RNA pro.	RNA processing and modification	INFORMATION STORAGE AND PROCESSING
Transcription	Transcription	INFORMATION STORAGE AND PROCESSING
Replication, recombi.	Replication, recombination and repair	INFORMATION STORAGE AND PROCESSING
Chromatin struc.	Chromatin structure and dynamics	INFORMATION STORAGE AND PROCESSING
Cell cycle.	Cell cycle control, cell division, chromosome partitioning	CELLULAR PROCESSES AND SIGNALING
Nuclear structure	Nuclear structure	CELLULAR PROCESSES AND SIGNALING
Defense mechanisms	Defense mechanisms	CELLULAR PROCESSES AND SIGNALING
Signal transduction.	Signal transduction mechanisms	CELLULAR PROCESSES AND SIGNALING
Cell wall/memb.	Cell wall/membrane/envelope biogenesis	CELLULAR PROCESSES AND SIGNALING
Cell motility	Cell motility	CELLULAR PROCESSES AND SIGNALING
Cytoskeleton	Cytoskeleton	CELLULAR PROCESSES AND SIGNALING
Extracellular structures	Extracellular structures	CELLULAR PROCESSES AND SIGNALING
Intracellular trafficking.	Intracellular trafficking, secretion, and vesicular transport	CELLULAR PROCESSES AND SIGNALING
Posttranslational modi.	Posttranslational modification, protein turnover, chaperones	CELLULAR PROCESSES AND SIGNALING
Energy production.	Energy production and conversion	METABOLISM
Carbohydrate transport.	Carbohydrate transport and metabolism	METABOLISM
A.A. transport.	Amino acid transport and metabolism	METABOLISM
Nucleotide transport.	Nucleotide transport and metabolism	METABOLISM
Coenzyme transport.	Coenzyme transport and metabolism	METABOLISM
Lipid transport.	Lipid transport and metabolism	METABOLISM
Inorganic ion.	Inorganic ion transport and metabolism	METABOLISM
Secondary metabolites.	Secondary metabolites biosynthesis, transport and catabolism	METABOLISM
General function.	General function prediction only	POORLY CHARACTERIZED
Function unknown	Function unknown	POORLY CHARACTERIZED

'Advanced Aggregate' is

Species

"Species" is a biological species selected by users from "Select Species".

Taxonomic Class

"Taxonomic Class" is a trivial name of classification (land plants, green algae, red algae, glaucophyceae, oomycetes, diatoms, and others).

Habitat

"Habitat" is a place where an organism makes its home (freshwater, marine, terrestrial, and ubiquitous).

Unicellular/Multicellular

"Unicellular/Multicellular" is a type of cell as an organism, single-celled organism or multi-celled organism, respectively.

Protein Cluster

"Protein Cluster" is a classification of species clustering with the OrthoMCL tool (Species specific: single-membered cluster, Common: all-membered cluster, and Other: others).

KOG

KOG (EuKaryotic Orthologous Groups) is a eukaryote-specific version of COG (The Clusters of Orthologous Groups) database for identifying ortholog and paralog proteins, which database include 25 functions

. Sometimes, there is a difference in hit counts between KOG and others, because KOG allows duplicated data.
go to KOG page

Arabidopsis thaliana
Physcomitrella patens
Selaginella moellendorffii
Klebsormidium flaccidum
Bathycoccus prasinos
Micromonas pusilla
Micromonas sp. RCC299
Ostreococcus tauri
Ostreococcus lucimarinus
Chlamydomonas reinhardtii
Monoraphidium neglectum
Volvox carteri
Auxenochlorella protothecoides
Chlorella variabilis
Coccomyxa subellipsoidea C-169
Chondrus crispus
Cyanidioschyzon merolae
Galdieria sulphuraria
Porphyridium purpureum
Pyropia yezoensis
Cyanophora paradoxa
Phytophthora capsici
Phytophthora infestans
Phytophthora ramorum
Phytophthora sojae
Fragilariopsis cylindrus CCMP 1102
Phaeodactylum tricornutum
Thalassiosira pseudonana
Aureococcus anophagefferens
Ectocarpus siliculosus
Symbiodinium minutum
Emiliania huxleyi
Guillardia theta
Bigelowiella natans

chlo
chlo_mito
cysk
cysk_plas
cyto
cyto_E.R.
cyto_nucl
cyto_plas
E.R.
E.R._plas
E.R._vacu
extr
golg
golg_plas
mito
mito_plas
nucl
nucl_plas
pero
plas
vacu